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Pz-1-based PROTACs activity on CCDC6-RET. TPC1 cells were treated with the indicated PROTACs or Pz-1 for 12 h at the indicated concentrations. Cells were harvested, and extracts were subjected to Western blotting with anti-RET antibody. Tubulin was used as a loading control.

Journal: Scientific Reports

Article Title: Discovery of a Proteolysis Targeting Chimera for TRKA and RET-derived oncoproteins

doi: 10.1038/s41598-025-25687-w

Figure Lengend Snippet: Pz-1-based PROTACs activity on CCDC6-RET. TPC1 cells were treated with the indicated PROTACs or Pz-1 for 12 h at the indicated concentrations. Cells were harvested, and extracts were subjected to Western blotting with anti-RET antibody. Tubulin was used as a loading control.

Article Snippet: Antibodies for VEGFR2 (2479), phospho-TRKA (Tyr490) (9141), phospho-TRKA (Tyr674/675) (4621), panTRK (A7H6R) (92991) and RET (14698) were from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activity Assay, Western Blot, Control

Compound 9 activity on RET- and TRKA-driven cell proliferation. The indicated cell lines were incubated with vehicle (NT: not treated) or increasing concentrations of compound 9 and counted at the indicated time points. Data are the mean ± SD of a single experiment performed in triplicate. Number of cells plated at Day 0: KM12 (10,000/well); TT (200,000/well); TPC-1 (5000/well); MZ-CRC-1 and Lc-2/ad (100,000/well). IC 50 dose was calculated using PRIZM software (GraphPad Software Inc). Confidence intervals are indicated in brackets.

Journal: Scientific Reports

Article Title: Discovery of a Proteolysis Targeting Chimera for TRKA and RET-derived oncoproteins

doi: 10.1038/s41598-025-25687-w

Figure Lengend Snippet: Compound 9 activity on RET- and TRKA-driven cell proliferation. The indicated cell lines were incubated with vehicle (NT: not treated) or increasing concentrations of compound 9 and counted at the indicated time points. Data are the mean ± SD of a single experiment performed in triplicate. Number of cells plated at Day 0: KM12 (10,000/well); TT (200,000/well); TPC-1 (5000/well); MZ-CRC-1 and Lc-2/ad (100,000/well). IC 50 dose was calculated using PRIZM software (GraphPad Software Inc). Confidence intervals are indicated in brackets.

Article Snippet: Antibodies for VEGFR2 (2479), phospho-TRKA (Tyr490) (9141), phospho-TRKA (Tyr674/675) (4621), panTRK (A7H6R) (92991) and RET (14698) were from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activity Assay, Incubation, Software